Femtonics FEMTO3D ATLAS all-in-one 3D microscope for two-photon microscopy

Femtonics’ FEMTO3D Atlas combines high-tech science and engineering in 3D measurements. It goes beyond traditional galvo and resonant scanner-based imaging functions by combining them with a novel fast 3D imaging feature, providing an all-in-one solution for two-photon microscopy. The device enables its users to scan cells and neuronal, dendritic or other biological processes in 3D, up to a million times faster than classical scanning methods, at a preserved two-photon resolution — making it suitable for network and dendritic imaging, behavioural studies and optogenetics.

Neural networks consist of thousands of neurons dispersed in 3D space, often across many cortical layers of the brain tissue. 3D random-access scanning performed by AO (acousto-optic) technology combined with two-photon microscopy can reveal the dynamics of the neural networks while processing information, enabling recording of neuronal activity from cell populations consisting of up to thousands of cells in vivo. FEMTO3D Atlas’s 3D anti-motion technology and scanning methods provide a flexible solution to gather motion-corrected data in large volumes at high frame rates during behaviour.

The product is available as a standalone microscope or as a 3D imaging extension for various types of existing systems. Other features include: simultaneous readout of neural activity on both the somatic and dendritic scales; increased measurement speed and signal-to-noise ratio by several orders of magnitude; 3D network imaging of over 2000 soma distributed in 3D; imaging of dendritic branches through layers; simultaneous imaging of over thousands of spines; flexible scanning methods supporting in vivo and in vitro measurements; in vivo real-time 3D motion correction; high-speed 3D random-access ROI scanning (scanning speed up to 100 kHz to any points in 3D); high-speed raster scanning of 40 fps at 510 x 510, and even 3000 fps at reduced FOV; good excitation efficiency for large 3D scanning volume with GECIs; automatic wavelength tuning between 750 and 1050 nm; functional imaging correlated with electrophysiological data; calcium and voltage imaging during behaviour; and 3D random-access photo stimulation for optogenetics.