Luxendo InVi SPIM Lattice Pro light sheet microscope for live cell imaging

Wednesday, 06 March, 2024 | Supplied by: SciTech Pty Ltd

The Luxendo InVi SPIM Lattice Pro light sheet microscope provides a high level of flexibility for live cell imaging. It illuminates samples using a proprietary Advanced Illumination Module (AIM), making it suitable for accessing the complex structure and behaviour of whole embryos, cell cultures and organoids. As a result, microscopy applications such as studies in embryogenesis and developmental biology, neurobiology and neurodevelopment, plant science, etc, receive high-quality results for 3D high-resolution imaging experiments — including large field of view, high speed and optimal spatial resolution.

The product offers a variety of illumination patterns, ranging from the classical static Gaussian light-sheet or a scanned Gaussian beam to sophisticated illumination schemes like Bessel beams, Airy beams or optical lattices. A broad range of beam shapes is offered to improve the microscope’s resolution and reduce photo-damage in delicate samples.

A Special Optics 28.6x 0.7 NA water immersion objective lens projects the light-sheet on the sample. A high numerical aperture Nikon CFI Apo 25x W 1.1 NA water immersion objective lens images the signal onto one or two Hamamatsu sCMOS cameras. An additional magnification changer provides 31.3x and 62.5x total magnification to allow the user to optimise field of view and pixel size to their experimental needs.

The light sheet microscope is claimed to capture high-resolution images faster, over longer acquisition times and with far less photodamage than other microscopes used in live cell studies. As a result, it is said to enable observation and precise tracking of highly complex and dynamic molecular and cellular events in an almost unlimited range of organisms, particularly during embryonic development and in 3D cell culture applications. These in vivo studies are integral to leading-edge developmental, cell biology and organoid research.

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