Thermo Scientific Q Exactive Focus LC-MS/MS

Laboratories performing food and environmental testing, clinical research, forensic toxicology, pharmaceutical/biopharmaceutical measurements and other applied analyses can now benefit from the performance of high-resolution accurate-mass (HRAM) Orbitrap mass spectrometry using an instrument designed for cost-per-sample-sensitive workflows.

The Thermo Scientific Q Exactive Focus LC-MS/MS is designed to make the qualitative and quantitative power of Orbitrap-based instruments available to users who use quadrupole time-of-flight (Q-TOF) mass spectrometry or other technologies. Besides comparable quantitative data for frequently detected compounds, it provides a complementary screen of many other compounds from the same injection.

The product combines high-performance quadrupole precursor ion selection with an HRAM Orbitrap mass analyser, providing good mass accuracy, sensitivity and fast polarity switching. This has been shown to selectively and accurately quantify and confirm analytes with comparable sensitivity to triple quadrupole instruments.

The unit is designed to deliver up to 70,000 resolution at m/z 200 and scan speed up to 12 Hz. Sub ppm mass accuracy has been achieved routinely in company and early-access user laboratories. The instrument is designed for quantitation experiments, with capabilities that include selected ion monitoring (SIM), parallel reaction monitoring (PRM) and data-independent acquisition (DIA).

Full scan confirmation mode and parallel reaction monitoring (PRM) are designed for targeted screening combined with reproducible quantitation results. Data-independent acquisition (DIA) provides qualitative coverage for screening unknowns with supporting quantitative data for many of the unknowns identified.