Amersham ECL detection reagent

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The Amersham ECL detection reagent uses enhanced luminol-based detection, suitable for all routine confirmatory Western blotting experiments. The product has for decades been a widely used chemiluminescent detection reagent for researchers worldwide, giving correct results and being cited in thousands of publications worldwide.

Based on the enhanced chemiluminescent reaction of luminol with horseradish peroxidase, ECL substrate can be used to detect probes which have been labelled either directly or indirectly with horseradish peroxidase. Rapid light output enables results to be achieved in 10 to 15 min. The substrate is useful for target amounts above 500 fg.

Offering high sensitivity and fast results, the product detects up to 10 to 12 ng of protein, which is said to be ~10x more than colorimetric methods. It is optimised to use with Amersham Protran nitrocellulose (NC) membranes and delivers results in just a few minutes, after which users can image using a CCD imager like the Amersham ImageQuant 500 or Amersham Hyperfilm ECL.

The convenient, easy-to-use kit contains ECL detection reagent, horseradish peroxidase (HRP)-conjugated antibodies and blocking agent.

For more information: https://cytiva.link/6xqt9.