ChromoTek Halo-Trap affinity beads for immunoprecipitation

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ChromoTek’s Halo-Trap is designed for immunoprecipitation of Halo fusion proteins and their interacting factors from cell or organelle extracts and lysates for biochemical analyses such as immunoprecipitation (IP)/co-IP, mass spectrometry and enzyme activity measurements. Also available, is the highly specific and selective anti-Halo monoclonal mouse antibody, designed for use in Western blotting.

Halo-Trap for immunoprecipitation is a single-domain antibody fragment, aka V_HH or Nanobody, coupled to beads for immunoprecipitation of Halo fusion proteins. The product immunoprecipitates Halo fusion proteins even when already covalently bound to Halo ligands, ie, after labelling, etc. It can be used for affinity purification and the bound Halo fusion protein can be eluted without protease.

Halo-Trap is a convenient and high-performing tool, ready to use, for fast and efficient one-step immunoprecipitation of Halo fusion proteins. It is fast and strong at binding, has low background levels and works under harsh conditions, enabling single-band purification of Halo fusion proteins. It is available coupled to Agarose, Magnetic Agarose and Magnetic Particles M-270, with Halo-Trap Agarose best for low background and high binding capacity; Halo-Trap Magnetic Agarose for magnetic separation and high binding capacity; and Halo-Trap Magnetic Particles M-270 for magnetic separation of large proteins.