AdipoGen Life Sciences APO-1-3 and APO-1-1 apoptosis-inducing antibodies

Programmed cell death plays a crucial role in development and host defence. Fas (CD95) is a member of the death receptor (DR) family, a subfamily of the tumour necrosis factor receptor superfamily. The formation of the Fas death-inducing signalling complex (DISC) is the initial step of Fas signalling.

Activation of procaspase-8 at the DISC leads to the induction of DR-mediated apoptosis. Stimulation of Fas has been also reported to trigger non-apoptotic pathways. It has been shown that membrane-bound FasL is essential for the cytotoxic activity, whereas soluble FasL appears to promote autoimmunity and tumorigenesis via induction of non-apoptotic pathways, particularly NF-κB.

AdipoGen Life Sciences’ anti-Fas clones APO-1-3 and APO-1-1 are monoclonal antibodies that recognise human Fas and are capable of inducing Fas-mediated apoptosis. For western blot and functional applications, anti-Fas (human), mAb (APO-1-3) is recommended. APO-1-1 (IgG₁) is an isotype-switch variant of the original clone APO-1-3 (IgG₃). Since undesired side reactions in immunohistochemistry are minimal for IgG1 antibodies, APO-1-1 is especially useful for immunohistology on cryostat sections and on cytospin preparations.