Bio-Rad Laboratories EveryBlot Blocking Buffer

Bio-Rad Laboratories has launch of the EveryBlot Blocking Buffer — a western blot blocking reagent that offers 5 min blocking time and produces high signal and low background levels. This is said to result in greater sensitivity for western blots compared to other available blocking buffers.

When attempting to detect a new protein target, researchers often optimise several variables including the use of specific blocking buffers (reagents) when performing western blots. For example, many blocking buffers are optimised for specific detection methods such as chemiluminescence or fluorescence detection and some buffers may not be compatible for detection of some targets.

EveryBlot offers high sensitivity and low background with both chemiluminescent and fluorescence detection across a wide range of targets. In addition, other blocking buffers require blocking times of up to an hour, while EveryBlot Blocking Buffer completes the blocking step in 5 min, shortening the duration of the immunodetection step.

The product is compatible with all detection methods as well as for detection of phosphorylated targets, which eliminates the need for optimisation. It also prevents non-specific binding to increase sensitivity.