Bio-Rad Laboratories Reliance One-Step Multiplex Supermix

Bio-Rad Laboratories has launched the Reliance One-Step Multiplex Supermix — a sensitive and robust reagent mix for detection and quantification of up to five RNA or DNA targets in one reaction.

One-step RT-qPCR–based applications are increasingly being used in life science research to quickly quantify genes of interest directly from many RNA samples simultaneously. The applications are used in particular and for virus/pathogen detection and for high-throughput screening.

Reliance One-Step Multiplex Supermix offers robust, sensitive amplification and detection of up to five targets under a wide range of conditions, including challenging templates, difficult targets or targets that are in the presence of PCR inhibitors. With its room-temperature stability, the supermix can be easily integrated into automated workflows.

The ready-to-use, single-tube reagent mix has been optimised for sensitive amplification of up to five targets in a single reaction. It is formulated with an optimal enzyme blend, including a Reliance reverse transcriptase and Sso7d fusion polymerase and an advanced buffer to maximise sensitivity and efficiency in multiplex RT-qPCR reactions. Its extended assembled reaction stability (up to 24 h) is adapted for high-throughput automated workflows.

The supermix was formulated to offer increased sensitivity and rapid results to address a wide range of RNA detection questions. When used as part of a workflow with Bio-Rad’s CFX Automation System II and PrimePCR Probe Assays, it is said to enable researchers to maximise throughput and quality of RT-qPCR data, processing 40 plates or more in 24 h.