BioLamina Biosilk biomaterial for 3D cell culture of hPSCs

BioLamina’s Biosilk is a 3D cell culture biomaterial made from recombinant spider silk protein. It is a biocompatible, biodegradable and non-immunogenic material that is suitable for integration, proliferation and subsequent lineage-specific differentiation of human pluripotent stem cells (hPSCs). The extendable macrofibre material can self-assemble into several formats: foams, fibre bundles and thin films.

Unlike many other 3D scaffolds, the cells can be added to the Biosilk solution before the network stabilises. This results in a uniform integration of the cells between the microfibres, where the cells can then effectively proliferate and migrate, resulting in full and even access to the medium, matrix and neighbouring cells.

The product is a flexible and non-immunogenic 3D culture system that can be assembled to accommodate different formats. It has elastic microfibres that mimic the extracellular matrix and is animal origin-free to the primary level. The material can be further functionalised with the user’s choice of BioLamina’s Biolaminin product, for improved cell functionality.