BioStatus HypoxiTRAK far-red fluorescent reporter of hypoxia

BioStatus’s HypoxiTRAK is a novel, far-red fluorescing dye that reveals the hypoxic experience of individual live cells. Non-toxic to normoxic cells, it is designed to enable entirely new assay approaches to functional hypoxia.

The hypoxia sensing range is relevant to biomarker and hypoxia-targeting drug development. It is suitable for studies over several days, and read-out is direct, with no requirement for cell processing. As no fixation is required, the product can provide a convenient negative selection for cells not experiencing significant hypoxia within heterogeneous populations, including 3D culture.

The dye can be used in flow cytometry and fluorescence imaging and is spectrally compatible with common visible range fluors including FITC and R-PE. In flow cytometry, it can be used to extract statistical information on the distribution of hypoxic experience over both time and treatment conditions. In fluorescence microscopy, it can assist in gaining an understanding of the spatial hypoxic experience in micro-tissues and spheroids.