EpigenTek EpiQuik In Vivo Protein Sumoylation Assay Ultra Kit (Colorimetric)

SUMOylation is a post-translational modification involved in various cellular processes, such as nuclear-cytosolic transport, transcriptional regulation, apoptosis, protein stability, response to stress and progression through the cell cycle. SUMO proteins are similar to ubiquitin, where SUMOylation is directed by an enzymatic cascade analogous to that involved in ubiquitination.

SUMOylation of target proteins in vivo has been shown to cause several different outcomes, including altered localisation and binding partners. Detection of in vivo protein SUMOylation (SUMO conjugation) would provide useful information for understanding SUMO modification that emerges as an important control mechanism regulating the activity of many nuclear proteins.

EpigenTek’s EpiQuik In Vivo Protein Sumoylation Assay Ultra Kit is a complete set of optimised reagents designed for measuring in vivo protein SUMOylation from multiple mammalian cells/tissues including human, mouse and rat. The kit can perform the direct colorimetric assay without the need for affinity chromatography or Western blot; has a flexible choice of antibody of interest that allows for the detection of SUMOylation of multiple target proteins simultaneously; uses optimised detection antibodies where it eliminates the step for detection solution preparation, thereby increasing detection sensitivity; and allows consistent assay conditions within 5 h.