Eppendorf Eporator for transforming microorganisms with DNA/RNA

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The Eporator can be used as a fast, simple and safe way to transform bacteria, yeast and other microorganisms with DNA/RNA. Electroporation is claimed to yield significantly higher transformation efficiency, with highly reproducible results, compared with chemical methods.

Exposing bacteria or yeast strains to short, high-voltage electrical pulses enables macromolecules, such as plasmid DNA, to diffuse into the cell through temporary pores in the cell membrane. Designed to deliver ideal conditions for electroporation of bacteria and yeast, the instrument is claimed to give transformation efficiencies 10 times higher than with chemical transformation (heat shock method) and 50% higher than the earlier Electroporator 25102.

Two program buttons allow storage and recall of most commonly used parameters and simple one-button operation allows faster sample handling.

The instrument has a compact design for easy storage and transport and comes with a USB port facilitating export of data for analysis and GLP-compliant documentation.

Application protocols for the electroporation of various bacteria and yeast strains can be found at www.eppendorf.com/eporator.