Abcam engineered knockout cell lysates

Abcam’s engineered knockout cell lysates enable proteomic studies by providing researchers with ‘true negative’ controls.

Acquiring off-the-shelf knockout mouse models or cell lines that match specific experimental requirements can be challenging, and so the products often need to be sourced directly from individual researchers or labs. Abcam’s latest collection addresses this issue by providing access to over 2800 diploid KO cell lysates.

The lysates are useful for studies requiring loss-of-function phenotypes at the proteomic level as well as routine applications such as Western blotting and mass spectroscopy. They also can be used to support antibody and target validation. The use of diploid cells makes the lysates well suited to the more complex studies often carried out in cancer research.

Derived from commonly used immortalised cell lines, KO lysates have been engineered using CRISPR-Cas9 and are accompanied by Sanger sequencing and Western blotting validation data. Corresponding wild-type controls are also provided so that the biological impact of each KO lysate can easily be assessed within a consistent cellular background.

Abcam offers access to thousands of CRISPR-Cas9-engineered KO cell lysates of interest, without needing to generate or purchase a KO cell line, saving on average 4–12 weeks’ work in the lab. Diploid KO lysates are derived from commonly used cell lines including HeLa, HEK293T, A549, HCT116, Hep G2 and MCF. There is minimal preparation required.