Integrated DNA Technologies RNA interference (RNAi) products

Integrated DNA Technologies (IDT) has a comprehensive range of customisable products for RNA interference (RNAi) applications. The Screening Dicer-substrate siRNA (DsiRNA) provides researchers with a quick solution for their RNAi requirements. IDT’s DsiRNAs are chemically synthesised 27-mer RNA duplexes optimised for Dicer processing and incorporation into the RISC complex, resulting in an increase in potency. IDT’s predesigned library includes over 3,000,000 DsiRNAs designed against human, mouse, rat, cow, dog, chicken and chimp transcriptomes within RefSeq, enabling the production of DsiRNAs to meet the majority of user requirements.

When developing predesigned DsiRNA duplexes, site selection is first performed using a proprietary algorithm with novel design rules. The sequences are subsequently screened to minimise the potential for cross-hybridisation and off-target effects, while sequences containing any known small nucleotide polymorphisms (SNPs) or alternatively spliced exons are eliminated.

IDT therefore offers two variants of predesigned DsiRNA duplexes: splice common and splice specific. Splice-common duplexes target all known variants of a gene in RefSeq and 8-10 duplexes are available for each target gene. Splice-specific duplexes target exons present in particular splice variants, making them suitable for researchers targeting specific isoforms.