Lonza L7 PBMC Reprogramming Bundle for the generation of hiPSCs from PBMCs

Wednesday, 24 June, 2015 | Supplied by: Lonza Australia Pty Ltd

Lonza has released the latest addition to its L7 hPSC System for the generation of human induced pluripotent stem cells (hiPSCs) and the maintenance of stem cells from a variety of sources. The L7 PBMC Reprogramming Bundle enables researchers to reprogram peripheral blood mononuclear cells (PBMCs) for use in downstream experiments. Because of its series of reagents and protocols optimised for working with PBMCs, the bundle makes it easy and fast to generate hiPSCs.

The L7 hPSC System offers a complete workflow that combines primary cells, reprogramming kits, transfection tools and culturing media, along with a matrix, passaging solution and cryosolution. It streamlines the reprogramming of somatic cells to generate hiPSCs, as well as supporting the ongoing maintenance and utilisation of human embryonic stem cells (hESCs) and hiPSCs.

The bundle contains a number of components, including the L7 PBMC Priming-Recovery Kit, which primes PBMCs prior to reprogramming and provides optimal recovery of the hiPSCs following the delivery of reprogramming vectors using Lonza’s 4D-Nucleofector technology. The bundle also contains PBMC-specific reprogramming enhancers for high-quality results. The company also offers a source of human PBMCs for researchers to use as a positive control donor during their reprogramming experiments.

Once the PBMCs have undergone the reprogramming process, they are cultured and expanded using the L7 hPSC Culture System, which includes the L7 hPSC Matrix, Basal Medium and Supplement. To assist with passaging these cells, the L7 hPSC Passaging Solution offers non-enzymatic cell detachment, without the need for mechanical manipulation. This method has been formulated to protect valuable cell lines. The L7 hPSC Cryosolution enables the efficient recovery of frozen cells after storage.

The L7 System is xeno-free, fully defined and said to support the long-term culture and pluripotency of PSCs. The hiPSCs created using the bundle can differentiate into all three germ layers and are ready for use across a range of applications, from basic research and disease modelling through to drug development and regenerative medicine.

Online: www.lonza.com
Phone: 03 9550 0883
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