The Moist Chamber type 834/8 from Harvard Apparatus is a flexible and useful tool for perfusion of organs, such as liver and kidney from typical rodent models.
In its most basic configuration, the chamber consists of a suitably deep (110 x 40 x 35 mm) organ chamber and tight-fitting cover. Both components are double walled and water jacketed to provide a stable, temperature-controlled environment within the organ chamber.
Physiological perfusion medium is warmed by passage through a built-in heat exchanger and bubble trap immediately before contact with the organ.
Inside the chamber, a flexible silicone platform acts as a rest for preparation and cannulation of the organ. Anchors for the Mini Ball Joint positioning system and precision arterial and venous cannulae are pre-drilled on both sides of the organ.
In addition, several measurement and sample ports are provided for easy access to the inner chamber even with the cover in place. This makes the chamber suitable for collecting a wide range of physiological data. The chamber can be part of a simple, constant flow perfusion system for testing potential vasoactive compounds, current used to form the core of a combined constant pressure/constant flow perfusion system for more complex perfusion and dose-response regimens.
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