PCR cycler

Monday, 10 August, 2009 | Supplied by: QIAGEN Pty Ltd

The versatile Rotor-Gene Q real-time PCR cycler features a centrifugal rotary design. Each tube spins in a chamber of moving air, keeping all samples at precisely the same temperature during rapid thermal cycling. Detection is similarly uniform.

When each tube aligns with the detection optics, the sample is illuminated and the fluorescent signal rapidly collected from a single, short optical pathway. This thermal and optical uniformity results in sensitive, precise, fast real-time PCR analysis. It also eliminates sample-to-sample variations and edge effects. These are unavoidable in traditional block-based instruments due to temperature gradients across the block and multiple, complex optical pathways.

The rotary design delivers: well-to-well variation below ±0.01°C, uniform detection eliminating the need for ROX reference dye and fast ramping and negligible equilibration times for short run times. With up to six channels spanning UV to infrared wavelengths, the cycler delivers a wide optical range.

The Rotor-Gene Q supports multiple PCR tube formats to suit a range of needs. As well as tubes, Rotor-Discs, circular plates of vertically oriented reaction wells, are available for accelerated set-up and higher throughput (96 wells with four reference wells).

Online: www.qiagen.com
Phone: 03 9840 9800
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