X-gal is a well-known reagent that has been used to detect β-galactosidase in cell or tissue samples. Cells or tissues in these assays, however, must be fixed due to poor cell permeability. In addition, assays using fluorescent reagents cannot clearly differentiate β-galactosidase-expressed cells or regions. Dojindo’s SPiDER-βGal is able to overcome these issues as it possesses cell permeability and the ability to be retained in the intracellular region.
Through an enzymatic reaction, the reagent immediately forms a quinone methide that acts as an electrophile when proteins containing nucleophilic functional groups are nearby. When the probe reacts with a protein, the conjugates become fluorescent compounds. Thus, the product allows single-cell analysis because it self-immobilises to the intracellular proteins.
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